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Researchers from MSU and KKU Find Pyrosequencing Approaches that are able to Discriminate among Diphyllobothriidean Species
In a paper published online 17 November 2016 in The Scientific Report, a research team led by Khon Kaen University and Mahasarakham University professors find pyrosequencing approaches that are able to discriminate among diphyllobothriidean species.
The results came out of collaboration between researchers from faculties of medicines of Mahasarakham University and Khon Kaen University consisting of Assistant Professor Dr. Tongjit Thanchomnang, Dr. Somjintana Tourtip, Professor Dr. Wanchai Maleewonga and others.
This study entitled “Rapid identification of nine species of diphyllobothriidean tapeworms by pyrosequencing” was supported by a TRF Senior Research Scholar Grant, Thailand Research Fund grant.
The identification of diphyllobothriidean tapeworms (Cestoda: Diphyllobothriidea) that infect humans and intermediate/paratenic hosts is extremely difficult due to their morphological similarities, particularly in the case of Diphyllobothrium and Spirometra species. A pyrosequencing method for the molecular identification of pathogenic agents has recently been developed, but as of yet there have been no reports of pyrosequencing approaches that are able to discriminate among diphyllobothriidean species. This study, therefore, set out to establish a pyrosequencing method for differentiating among nine diphyllobothriidean species, Diphyllobothrium dendriticum, Diphyllobothrium ditremum, Diphyllobothrium latum, Diphyllobothrium nihonkaiense, Diphyllobothrium stemmacephalum, Diplogonoporus balaenopterae, Adenocephalus pacificus, Spirometra decipiens and Sparganum proliferum. This method was found to be a reliable tool not only for species identification of diphyllobothriids, but also for epidemiological studies of cestodiasis caused by diphyllobothriidean tapeworms at public health units in endemic areas.
See more at: http://www.web.msu.ac.th/ssystem/msuhotnews/detailnews.php?hm=&hotnewsid=7096&uf=&qu=